Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED.

ABSTRACT: The lipidic cubic phase (LCP) technique has proved to facilitate the growth of high-quality crystals that are otherwise difficult to grow by other methods. However, the crystal size optimization process could be time and resource consuming, if it ever happens. Therefore, improved techniques for structure determination using these small crystals is an important strategy in diffraction technology development. Microcrystal electron diffraction (MicroED) is a technique that uses a cryo-transmission electron microscopy to collect electron diffraction data and determine high-resolution structures from very thin micro- and nanocrystals. In this work, we have used modified LCP and MicroED protocols to analyze crystals embedded in LCP converted by 2-methyl-2,4-pentanediol or lipase, including Prote

SUBMITTER: Zhu L 

PROVIDER: S-EPMC7544639 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature