What Makes Thienoguanosine an Outstanding Fluorescent DNA Probe?

ABSTRACT: Thienoguanosine (^thG) is an isomorphic guanosine (G) surrogate that almost perfectly mimics G in nucleic acids. To exploit its full potential and lay the foundation for future applications, 20 DNA duplexes, where the bases facing and neighboring ^thG were systematically varied, were thoroughly studied using fluorescence spectroscopy, molecular dynamics simulations, and mixed quantum mechanical/molecular mechanics calculations, yielding a comprehensive understanding of its photophysics in DNA. In matched duplexes, ^thG's hypochromism was larger for flanking G/C residues but its fluorescence quantum yield (QY) and lifetime values were almost independent of the flanking bases. This was attributed to high duplex stability, which maintains a steady orientation and

SUBMITTER: Kuchlyan J 

PROVIDER: S-EPMC7544670 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature