Mandibular undifferentiated pleomorphic sarcoma: Molecular analysis of a primary cell population.

ABSTRACT:

Background

Undifferentiated pleomorphic sarcomas are one of the most common subtypes of soft tissue sarcomas. These are aggressive mesenchymal tumors and are devoid of the major known biomarkers except vimentin. Our objective was to establish and characterize a primary cell population from a mandibular UPS specimen.

Methods

The tumor was surgically removed from the right mandible of a 24-year-old male with IRB approved signed consent. Tumor was dissected, cultured ex vivo, and a cell population, MUPS-1, were isolated from outgrowths. Gene and protein expression profiles of both the primary tumor and the derived there from cells were obtained by quantitative RT-PCR and immunohistochemistry and included markers of epithelial, endothelial, and mesenchymal differentiation. To be

SUBMITTER: Amm HM 

PROVIDER: S-EPMC7545231 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature