RNA Identification of PRIME Cells Predicting Rheumatoid Arthritis Flares.

ABSTRACT:

Background

Rheumatoid arthritis, like many inflammatory diseases, is characterized by episodes of quiescence and exacerbation (flares). The molecular events leading to flares are unknown.

Methods

We established a clinical and technical protocol for repeated home collection of blood in patients with rheumatoid arthritis to allow for longitudinal RNA sequencing (RNA-seq). Specimens were obtained from 364 time points during eight flares over a period of 4 years in our index patient, as well as from 235 time points during flares in three additional patients. We identified transcripts that were differentially expressed before flares and compared these with data from synovial single-cell RNA-seq. Flow cytometry and sorted-blood-cell RNA-seq in additional patients were used to vali

SUBMITTER: Orange DE 

PROVIDER: S-EPMC7546156 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature