Solubility, Stability, and Avidity of Recombinant Antibody Fragments Expressed in Microorganisms.

ABSTRACT: Solubility of recombinant proteins (i.e., the extent of soluble versus insoluble expression in heterogeneous hosts) is the first checkpoint criterion for determining recombinant protein quality. However, even soluble proteins often fail to represent functional activity because of the involvement of non-functional, misfolded, soluble aggregates, which compromise recombinant protein quality. Therefore, screening of solubility and folding competence is crucial for improving the quality of recombinant proteins, especially for therapeutic applications. The issue is often highlighted especially in bacterial recombinant hosts, since bacterial cytoplasm does not provide an optimal environment for the folding of target proteins of mammalian origin. Antibody fragments, such as single-chain variable

SUBMITTER: Kang TH 

PROVIDER: S-EPMC7546209 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature