Spike-Stalk Injection Method Causes Extensive Phenotypic and Genotypic Variations for Rice Germplasm.

ABSTRACT: Genetic diversities or favorable genes within distantly related species are the important resources for crop genetic improvement and germplasm innovation. Spike-Stalk injection method (SSI) has long been applied in rice genetic improvement by directly introducing genetic materials from non-mating donor species, while its inheritance patterns and the underlying mechanisms are poorly elucidated. In this study, a rice variant ERV1 with improved yield-related traits was screened out in the way of introducing genomic DNA of Oryza eichingeri (2n=24, CC genome) into RH78 (Oryza sativa L. 2n=24, AA genome) using SSI method. Genome-wide comparison revealed that the genomic heterozygosity of ERV1 was approximately 8-fold higher than RH78. Restriction-site as

SUBMITTER: Hu Y 

PROVIDER: S-EPMC7546333 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature