A novel assay for drug screening that utilizes the heat shock response of Caenorhabditis elegans nematodes.

ABSTRACT: Biological organisms respond to environmental stressors by recruiting multiple cellular cascades that act to mitigate damage and ultimately enhance survival. This implies that compounds that interact with any of those pathways might improve organism's survival. Here, we report on an initial attempt to develop a drug screening assay based on the heat shock (HS) response of Caenorhabditis elegans nematodes. The protocol works by subjecting the worms to two HS conditions in the absence/presence of the test compounds. Post-heat shock survival is quantified manually or in semi-automatic manner by analyzing z-stack pictures. We blindly screened a cassette of 72 compounds in different developmental stages provided by Eli Lilly through their Open Innovation Drug Discovery program. The analysis ind

SUBMITTER: Chen CH 

PROVIDER: S-EPMC7546469 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature