Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening.

ABSTRACT: Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings. For complete details on the use and execution of this protocol, please refer to Phan et al. (2019).

SUBMITTER: Nguyen HTL 

PROVIDER: S-EPMC7546514 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature