Plasmid diversity among genetically related Klebsiella pneumoniae bla_KPC-2 and bla_KPC-3 isolates collected in the Dutch national surveillance.

ABSTRACT: Carbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention it is important to track the spread of K. pneumoniae and its plasmids between patients. Therefore, the major aim was to recapitulate the contents and diversity of the plasmids of genetically related K. pneumoniae strains harboring the beta-lactamase gene bla_KPC-2 or bla_KPC-3 to determine their dissemination in the Netherlands and the former Dutch Caribbean islands from 2014 to 2019. Next-generation sequencing was combined with long-read third-generation sequencing to reconstruct 22 plasmids. wgMLST revealed five genetic clusters comprised of K. pneumoniae bla_KPC-2 isolates and four clusters consisted of bla

SUBMITTER: Hendrickx APA 

PROVIDER: S-EPMC7546619 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature