Automated quantification of Candida albicans biofilm-related phenotypes reveals additive contributions to biofilm production.

ABSTRACT: Biofilms are organized communities of microbial cells that promote persistence among bacterial and fungal species. Biofilm formation by host-associated Candida species of fungi occurs on both tissue surfaces and implanted devices, contributing to host colonization and disease. In C. albicans, biofilms are built sequentially by adherence of yeast to a surface, invasion into the substrate, the formation of aerial hyphal projections, and the secretion of extracellular matrix. Measurement of these biofilm-related phenotypes remains highly qualitative and often subjective. Here, we designed an informatics pipeline for quantifying filamentation, adhesion, and invasion of Candida species on solid agar media and utilized this approach to determine the importance of these component phenotypes to C.

SUBMITTER: Dunn MJ 

PROVIDER: S-EPMC7547077 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature