Differential miRNA Expression: Signature for Glaucoma in Pseudoexfoliation.

ABSTRACT:

Purpose

To investigate the microRNA (miRNA) profile in patients with different stages of pseudoexfoliation (PXF).

Methods

Peripheral blood of patients with PXF (naïve to medical therapy and with no systemic disease/drugs) with ocular hypertension (OHT) and pseudoexfoliation glaucoma (PXG) was evaluated in triplicate for miRNA profiling using polymerase chain reaction (PCR) arrays. Those identified in the discovery stage were validated with evaluation of serum transforming growth factor-β1 (TGF-β1) levels by ELISA. The downstream targets of TGF-β1 and unfolded protein response (UPR) were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Predicted targets of the identified miRNA and KEGG pathway analysis were done using miRbase and DIANA to

SUBMITTER: Rao A 

PROVIDER: S-EPMC7547124 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature