Aβ oligomer induced cognitive impairment and evaluation of ACU193-MNS-based MRI in rabbit.

ABSTRACT:

Introduction

Amyloid-beta oligomers (AβOs) accumulate in Alzheimer's disease and may instigate neuronal pathology and cognitive impairment. We examined the ability of a new probe for molecular magnetic resonance imaging (MRI) to detect AβOs in vivo, and we tested the behavioral impact of AβOs injected in rabbits, a species with an amino acid sequence that is nearly identical to the human sequence.

Methods

Intracerebroventricular (ICV) injection with stabilized AβOs was performed. Rabbits were probed for AβO accumulation using ACUMNS (an AβO-selective antibody [ACU193] coupled to magnetic nanostructures). Immunohistochemistry was used to verify AβO presence. Cognitive impairment was evaluated using object location and object recognition memory tests and trace eyeblink conditi

SUBMITTER: Rozema NB 

PROVIDER: S-EPMC7547311 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature