Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8⁺ T cell activation in fluorinated ethylene propylene cell culture bags.

ABSTRACT:

Background

A major obstacle to anti-viral and -tumor cell vaccination and T cell immunotherapy is the ability to produce dendritic cells (DCs) in a suitable clinical setting. It is imperative to develop closed cell culture systems to accelerate the translation of promising DC-based cell therapy products to the clinic. The objective of this study was to investigate whether viral antigen-loaded monocyte-derived DCs (Mo-DCs) capable of eliciting specific T cell activation can be manufactured in fluorinated ethylene propylene (FEP) bags.

Methods

Mo-DCs were generated through a protocol applying cytokine cocktails combined with lipopolysaccharide or with a CMV viral peptide antigen in conventional tissue culture polystyrene (TCPS) or FEP culture vessels. Research-scale (< 10 mL)

SUBMITTER: Bastien JP 

PROVIDER: S-EPMC7547414 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature