A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples.

ABSTRACT:

Background

Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference protocol for the efficient generation of transcriptome data from growing number of samples. Here, a comprehensive methodology on how to preserve, extract, amplify, and sequence full-length mRNA transcripts from Plasmodium-infected blood samples is presented that can be fully streamlined for high-throughput studies.

Results

The utility of various commercially available RNA-preserving reagents in a range of storage conditions was evaluated. Similarly, several RNA extract

SUBMITTER: Kucharski M 

PROVIDER: S-EPMC7547485 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature