MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases.

ABSTRACT: Severity assessment of animal experiments is mainly conducted by using subjective parameters. A widely applicable biomarker to assess animal distress could contribute to an objective severity assessment in different animal models. Here, the distress of three murine animal models for gastrointestinal diseases was assessed by multiple behavioral and physiological parameters. To identify possible new biomarkers for distress 750 highly conserved microRNAs were measured in the blood plasma of mice before and after the induction of pancreatitis. Deregulated miRNA candidates were identified and further quantified in additional animal models for pancreatic cancer and cholestasis. MiR-375 and miR-203 were upregulated during pancreatitis and down regulated during cholestasis, whereas miR-132 was upr

SUBMITTER: Kumstel S 

PROVIDER: S-EPMC7547723 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature