Optimized sample preparation for fecal volatile organic compound analysis by gas chromatography-mass spectrometry.

ABSTRACT:

Introduction

Headspace gas chromatography-mass spectrometry (HS-GC-MS) is widely considered the gold standard of quantitative fecal VOC analysis. However, guidelines providing general recommendations for bioanalytical method application in research and clinical setting are lacking.

Objectives

To propose an evidence-based research protocol for fecal VOC analysis by HS-GC-MS, based on extensive testing of instrumental and sampling conditions on detection and quantification limits, linearity, accuracy and repeatability of VOC outcome.

Methods

The influence of the following variables were assessed: addition of different salt solutions, injection temperature, injection speed, injection volume, septum use, use of calibration curves and fecal sample mass. Ultimately, the opt

SUBMITTER: El Manouni El Hassani S 

PROVIDER: S-EPMC7547966 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature