Ericoid mycorrhizal symbiosis: theoretical background and methods for its comprehensive investigation.

ABSTRACT: Despite decades of intensive research (especially from 1970s to 1990s), the ericoid mycorrhizal (ErM) hair root is still largely terra incognita and this simplified guide is intended to revive and promote the study of its mycobiota. Basic theoretical knowledge on the ErM symbiosis is summarized, followed by practical advices on Ericaceae root sample collection and handling, microscopic observations and photo-documentation of root fungal colonization, mycobiont isolation, maintenance and identification and resynthesis experiments with ericoid plants. The necessity of a proper selection of the root material and its surface sterilization prior to mycobiont isolation is stressed, together with the need of including suitable control treatments in inoculation experiments. The culture-dependent a

SUBMITTER: Vohnik M 

PROVIDER: S-EPMC7548138 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature