Molecular sex identification of Malaysian White-Nest Swiftlet (Aerodramus fuciphagus Thunberg, 1812).
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ABSTRACT: The difficulty in differentiating the sex of monomorphic bird species has made molecular sexing an important tool in addressing this problem. This method uses noninvasively collected materials such as feathers and may be advantageous for sexing endangered as well as commercialized bird species. In this study, seven primer sets for sexing birds were screened in Aerodramus fuciphagus using a total of 13 feather samples that were randomly selected from the state of Perak, Malaysia. From the screening analysis, only one primer set (P8/WZ/W) successfully differentiated the sex of A. fuciphagus. PCR amplification produced a single 255-bp DNA fragment for males which was derived from CHD-Z (CHD gene region in the sex chromosome Z), while for the females it produced two fragme
SUBMITTER: Osman MA
PROVIDER: S-EPMC7548184 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
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