Design, Expression and Purification of Strongyloides stercoralis IgG4 Immunoreactive Protein (NIE) in Escherichia coli.

ABSTRACT:

Background

Strongyloidiasis is a public health concern in northern regions of Iran, caused by Strongyloides stercoralis. Auto-infection cycle can be resulted in high parasitic load, especially in immunocompromised hosts. Because of low sensitivity of stool culture and stool-based microscopy techniques, detection of antibodies in patient's sera can be an alternative diagnostic technique for detection of the nematode. In the present study, as the first step of the development of an ELISA kit for the detection of antibodies against the nematode, IgG4 immunoreactive protein (NIE) was expressed in Escherichia coli expression system, purified and verified.

Methods

The NIE gene sequence was retrieved from the GenBank. This sequence was codon-optimized for the e

SUBMITTER: Dastan K 

PROVIDER: S-EPMC7548455 | biostudies-literature | 2020 Jul-Sep

REPOSITORIES: biostudies-literature