Proteome analysis of Saccharomyces cerevisiae after methyl methane sulfonate (MMS) treatment.

ABSTRACT: The treatment of methyl methane sulfonate (MMS) increases sensitivity to the DNA damage which, further leads to the cell death followed by a cell cycle delay. Delay in the cell cycle is because of the change in global transcription regulation which results into proteome change. There are several microarray studies on the transcriptome changes after MMS treatment, but very few studies are reported related to proteome change. The proteome analysis in this report identified subgroups of proteins, belonging to known cell cycle regulators, metabolic pathways and protein folding. About 53 proteins were identified by MS/MS and found that 36 of them were induced, 10 were repressed and few of them showed insignificant change. Our results indicated the change in the interactome as well as phosphoryl

SUBMITTER: Bharati AP 

PROVIDER: S-EPMC7548944 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature