Interleukin-36γ-producing macrophages drive IL-17-mediated fibrosis.

ABSTRACT: Biomaterials induce an immune response and mobilization of macrophages, yet identification and phenotypic characterization of functional macrophage subsets in vivo remain limited. We performed single-cell RNA sequencing analysis on macrophages sorted from either a biologic matrix [urinary bladder matrix (UBM)] or synthetic biomaterial [polycaprolactone (PCL)]. Implantation of UBM promotes tissue repair through generation of a tissue environment characterized by a T helper 2 (T_H2)/interleukin (IL)-4 immune profile, whereas PCL induces a standard foreign body response characterized by T_H17/IL-17 and fibrosis. Unbiased clustering and pseudotime analysis revealed distinct macrophage subsets responsible for antigen presentation, chemoattraction, and phagocytosis, as well a

SUBMITTER: Sommerfeld SD 

PROVIDER: S-EPMC7549193 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature