Optimization and characterization of position-selective labelling of RNA (PLOR) for diverse RNA and DNA sequences.

ABSTRACT: Modifications of short RNAs at specific sites can be achieved commercially by solid-phase chemical synthesis method. However, labelling long RNAs is still challenging for the routine methods. Position-selective Labelling of RNA (PLOR) is a hybrid phase transcription method that allows to label RNAs at desired sites with great flexibility and decent efficiency. In principle, PLOR is a promising method for synthesis of long modified RNAs that are unable to be generated by solid-phase chemical synthesis and other methods. However, as a recently developed method, PLOR has been only applied to label a 71nt and a 104nt RNA, and the limited sequence applications of PLOR may hinder its potential usages. To extend PLOR to more RNAs, we tested the PLOR performances for various RNA sequences. Conside

SUBMITTER: Zhang X 

PROVIDER: S-EPMC7549629 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature