G₂-quadruplex in the 3'UTR of IE180 regulates Pseudorabies virus replication by enhancing gene expression.

ABSTRACT: RNA secondary structure elements in the mRNA 3'-untranslated regions (3'UTR) play important roles in post-transcriptional regulation. RNA structure elements in the viral RNA provide valuable model for studying diverse regulation mechanisms. Herpesvirus genomes are double-stranded DNA with GC-rich sequences, which can be transcribed into abundant GC-rich RNAs. It is valuable to explore the structures and function of those GC-rich RNAs. We identified a G₂-quadruplex-forming sequence named PQS18-1 in the 3'UTR of the unique immediate early gene of Pseudorabies virus (PRV), an important member of Alphaherpesvirinae subfamily. The RNA PQS18-1 was folded into parallel G-quadruplex structure, enhancing gene expression. Both non-G-quadruplex mutant and G₃-quadruplex mu

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7549663 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature