Tautomeric Equilibria of Nucleobases in the Hachimoji Expanded Genetic Alphabet.

ABSTRACT: Evolution has yielded biopolymers that are constructed from exactly four building blocks and are able to support Darwinian evolution. Synthetic biology aims to extend this alphabet, and we recently showed that 8-letter (hachimoji) DNA can support rule-based information encoding. One source of replicative error in non-natural DNA-like systems, however, is the occurrence of alternative tautomeric forms, which pair differently. Unfortunately, little is known about how structural modifications impact free-energy differences between tautomers of the non-natural nucleobases used in the hachimoji expanded genetic alphabet. Determining experimental tautomer ratios is technically difficult, and so, strategies for improving hachimoji DNA replication efficiency will benefit from accurate computationa

SUBMITTER: Eberlein L 

PROVIDER: S-EPMC7549732 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature