Insights into idarubicin antimicrobial activity against methicillin-resistant Staphylococcus aureus.

ABSTRACT:

Background

MRSA is a major concern in community settings and in health care. The emergence of biofilms and persister cells substantially increases its antimicrobial resistance. It is very urgent to develop new antimicrobials to solve this problem.

Objective

Idarubicin was profiled to assess its antimicrobial effects in vitro and in vivo, and the underlying mechanisms.

Methods

We investigated the antimicrobial effects of idarubicin against MRSA by time-kill analysis. The antibiofilm efficacy of idarubicin was assessed by crystal violet and XTT staining, followed by laser confocal microscopy observation. The mechanisms underlying the antimicrobial effects were studied by transmission electron microscopy, all-atom molecular dynamic simulations, SYTOX staini

SUBMITTER: She P 

PROVIDER: S-EPMC7549941 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature