Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I.

ABSTRACT: Metacyclogenesis is one of the most important processes in the life cycle of Trypanosoma cruzi. In this stage, noninfective epimastigotes become infective metacyclic trypomastigotes. However, the transcriptomic changes that occur during this transformation remain uncertain. Illumina RNA-sequencing of epimastigotes and metacyclic trypomastigotes belonging to T. cruzi DTU I was undertaken. Sequencing reads were aligned and mapped against the reference genome, differentially expressed genes between the two life cycle stages were identified, and metabolic pathways were reconstructed. Gene expression differed significantly between epimastigotes and metacyclic trypomastigotes. The cellular pathways that were mostly downregulated during metacyclogenesis involved glucose energy metab

SUBMITTER: Cruz-Saavedra L 

PROVIDER: S-EPMC7549971 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature