A novel biosafety level 2 compliant tuberculosis infection model using a Δ<i>leuD</i>Δ<i>panCD</i> double auxotroph of <i>Mycobacterium tuberculosis</i> H37Rv and <i>Galleria mellonella</i>.

ABSTRACT: Mammalian infection models have contributed significantly to our understanding of the host-mycobacterial interaction, revealing potential mechanisms and targets for novel antimycobacterial therapeutics. However, the use of conventional mammalian models such as mice, are typically expensive, high maintenance, require specialized animal housing, and are ethically regulated. Furthermore, research using Mycobacterium tuberculosis (MTB), is inherently difficult as work needs to be carried out at biosafety level 3 (BSL3). The insect larvae of Galleria mellonella (greater wax moth), have become increasingly popular as an infection model, and we previously demonstrated its potential as a mycobacterial infection model using Mycobacterium bovis BCG. Here we present a novel BSL2

SUBMITTER: Asai M 

PROVIDER: S-EPMC7550006 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature