Positive control synthesis method for COVID-19 diagnosis by one-step real-time RT-PCR.

ABSTRACT:

Backgrounds

The coronavirus disease 2019 (COVID-19) pandemic is still ongoing. Real-time reverse transcription polymerase chain reaction (real-time RT-PCR) is regarded as a gold-standard method for the diagnosis of COVID-19. However, unexpected contamination of synthesized positive control samples included in COVID-19 test kits have increased the inconclusiveness of disease interpretation. Therefore, it is important to establish new methods for the preparation of reliable positive controls that are not affected by contamination for the accurate for diagnosis of COVID-19, but it still remains a challenge.

Methods

A new approach for producing synthetic positive controls using synthetic positive template (SPT) oligonucleotides was designed. SPT oligonucleotides contain probe bi

SUBMITTER: Cho H 

PROVIDER: S-EPMC7550048 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature