Amixicile depletes the ex vivo periodontal microbiome of anaerobic bacteria.

ABSTRACT:

Objectives

Although periodontal diseases result from overgrowth of anaerobic bacteria, the effect of a specific knockdown of anaerobes on the disease outcome has yet to be examined. We have reported that amixicile, a non-toxic, readily bioavailable, and novel antimicrobial, specifically targets selected oral anaerobes through inhibition of the activity of pyruvate ferredoxin oxidoreductase (PFOR), a major enzyme mediating oxidative decarboxylation of pyruvate.

Methods

Here, we generated an ex vivo microbiome derived from gingival pockets of human subjects with chronic periodontal disease and evaluated the efficacy of amixicile in generating a specific knockdown of anaerobic bacteria present in the microbiome.

Results

Our bioinformatics analysis identified PFOR-like co

SUBMITTER: Gui Q 

PROVIDER: S-EPMC7550193 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature