Flow Cytometry-Based Protocols for the Analysis of Human Plasma Cell Differentiation.

ABSTRACT: Humoral immunity is established after differentiation of antigen-specific B cells into plasma cells (PCs) that produce antibodies of relevant specificities. Defects in the development, activation, or differentiation of B cells severely compromises the immune response. Primary immunodeficiencies are often characterized by hypogammaglobulinemia and the inability to mount effective antigen-specific antibody responses, resulting in increased susceptibility to infections. After IgA deficiency, which is most often asymptomatic, common variable immunodeficiency (CVID) is the most prevalent symptomatic primary immunodeficiency, but in most cases the underlying genetic causes are unknown or their roles in disease pathogenesis are poorly understood. In this study, we developed a protocol for in v

SUBMITTER: Khoenkhoen S 

PROVIDER: S-EPMC7550473 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature