Evidence of metabolic activity during low-temperature ovarian tissue preservation in different media.

ABSTRACT:

Purpose

Although ovarian tissue transportation has been validated for up to 24 h, there is no standard protocol to date. We aimed to elucidate how existing media currently used for ovarian tissue transportation affect ovarian tissue metabolism and cell viability.

Methods

Cow ovarian fragments were immersed in 0.9% NaCl solution, IVF medium, Leibovitz 15 medium (L-15), or PBS for 1, 4, or 24 h at 4 °C. Media were analyzed for pH, lactate dehydrogenase (LDH) activity, and glucose, pyruvate, and lactate concentrations, while apoptosis was assessed by TUNEL assays in fixed fragments. Viability rates were assessed by flow cytometry (FACS).

Results

There were lower pH levels in NaCl at all time points compared with other media. LDH activity increased with time and was lowes

SUBMITTER: Vilela JMV 

PROVIDER: S-EPMC7550475 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature