Prediction of major microRNAs in follicular fluid regulating porcine oocyte development.

ABSTRACT:

Purpose

The aim of the present study was to identify key microRNAs (miRNAs) in porcine follicular fluid (FF) that regulate oocyte growth.

Methods

miRNAs contained in FF were determined by small RNA-seq of exosome RNA. Upstream regulator miRNA was determined by ingenuity pathway analysis using differentially expressed genes in granulosa cells (GCs) between small follicles (1-2 mm in diameter) and large follicles (3-5 mm), and between follicles containing oocytes of high developmental ability and follicles containing oocytes of low developmental ability. The candidate miRNAs overlapping among the three miRNAs group were determined. Lastly, the effect of supplementation with FF, exosome-depleted FFs, or each miRNA on in vitro oocyte growth was examined.

Results

The miRNA

SUBMITTER: Inoue Y 

PROVIDER: S-EPMC7550524 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature