PAG1 directs SRC-family kinase intracellular localization to mediate receptor tyrosine kinase-induced differentiation.

ABSTRACT: All receptor tyrosine kinases (RTKs) activate similar downstream signaling pathways through a common set of effectors, yet it is not fully understood how different receptors elicit distinct cellular responses to cause cell proliferation, differentiation, or other cell fates. We tested the hypothesis that regulation of SRC family kinase (SFK) signaling by the scaffold protein, PAG1, influences cell fate decisions following RTK activation. We generated a neuroblastoma cell line expressing a PAG1 fragment that lacks the membrane-spanning domain (PAG1^TM-) and localized to the cytoplasm. PAG1^TM- cells exhibited higher amounts of active SFKs and increased growth rate. PAG1^TM- cells were unresponsive to TRKA and RET signaling, two RTKs that induce neuronal differe

SUBMITTER: Foltz L 

PROVIDER: S-EPMC7550700 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature