Identification of Disease-Associated Variants by Targeted Gene Panel Resequencing in Parkinson's Disease.

ABSTRACT: Background: Recent advanced technologies, such as high-throughput sequencing, have enabled the identification of a broad spectrum of variants. Using targeted-gene-panel resequencing for Parkinson's disease (PD)-associated genes, we have occasionally found several single-nucleotide variants (SNVs), which are thought to be disease-associated, in PD patients. To confirm the significance of these potentially disease-associated variants, we performed genome association analyses, using next-generation target resequencing, to evaluate the associations between the identified SNVs and PD. Methods: We obtained genomic DNA from 766 patients, who were clinically diagnosed with PD, and 336 healthy controls, all of Japanese origin. All data were analyzed using Ion AmpliSeq panel sequences,

SUBMITTER: Daida K 

PROVIDER: S-EPMC7550729 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature