Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides.

ABSTRACT: Synthetic biology holds promise to revolutionize the life sciences and biomedicine via expansion of macromolecular diversity outside the natural chemical space. Use of non-canonical amino acids (ncAAs) via codon reassignment has found diverse applications in protein structure and interaction analysis, introduction of post-translational modifications, production of constrained peptides, antibody-drug conjugates, and novel enzymes. However, simultaneously encoding multiple ncAAs in vivo requires complex engineering and is sometimes restricted by the cell's poor uptake of ncAAs. In contrast the open nature of cell-free protein synthesis systems offers much greater freedom for manipulation and repurposing of the biosynthetic machinery by controlling the level and identity of translation

SUBMITTER: Cui Z 

PROVIDER: S-EPMC7550873 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature