SLC2A3 promotes macrophage infiltration by glycolysis reprogramming in gastric cancer

ABSTRACT:

Background

Tumors display a high rate of glucose metabolism and the SLC2A (also known as GLUT) gene family may be central regulators of cellular glucose uptake. However, roles of SLC2A family in mechanism of metabolite communication with immunity in gastric cancer remains unknown.

Methods

Bioinformatics analysis and IHC staining were used to reveal the expression of SLC2A3 in gastric cancer and the correlation with survival prognosis. Real-time PCR, western blots, OCR, ECAR, lactate production and glucose uptake assays were applied to determine the effect of SLC2A3 on glycolysis reprogramming. We then investigated the consequences of SLC2A3 upregulation or inhibition on aerobic glycolysis, also explored the underlying mechanism. Bioinformatics analysis and in vitro and in

SUBMITTER: Yao X 

PROVIDER: S-EPMC7552479 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature