YTHDF2 Regulates Maternal Transcriptome Degradation and Embryo Development in Goat.

ABSTRACT: Maternal mRNA clearance is critical for the early embryo development, which is under the tight control of RNA N6-methyladenosine (m⁶A). However, little information is known regarding the maternal mRNA clearance and mechanisms behind it in farm animals. In the present study, 3362 differentially expressed genes (DEGs) were found during the maternal-to-zygotic transition (MZT) and determined as maternal mRNAs in goat. Of which, 1961 was decreased at the 4-cell stage embryos, while 1401 was trigged down-regulation at the 8-cell stage embryos, which were termed as maternally encoded mRNA decay genes and zygotic genome activation (ZGA)-dependent maternal mRNAs, respectively. The expression of m⁶A reader YTHDF2 was increased during goat ZGA, and knockdown of YTHDF2 resulted

SUBMITTER: Deng M 

PROVIDER: S-EPMC7552740 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature