Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model.

ABSTRACT: In vitro adipose tissue models can be used to provide insight into fundamental aspects of adipose physiology. These systems may serve as replacements for animal models, which are often poor predictors of obesity and metabolic diseases in humans. Adipose tissue consists of a rich vasculature that is essential to its function. However, the study of endothelial cell-adipocyte interactions has been challenging due to differences in culture conditions required for the survival and function of each cell type. To address this issue, we performed an extensive evaluation of the cell culture media composition to identify the conditions optimal for the co-culture of endothelial cells and adipocytes. The effects of individual media factors on cell survival, proliferation, and differentiation were syst

SUBMITTER: Yang F 

PROVIDER: S-EPMC7552791 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature