Exploring the sequence fitness landscape of a bridge between protein folds.

ABSTRACT: Most foldable protein sequences adopt only a single native fold. Recent protein design studies have, however, created protein sequences which fold into different structures apon changes of environment, or single point mutation, the best characterized example being the switch between the folds of the GA and GB binding domains of streptococcal protein G. To obtain further insight into the design of sequences which can switch folds, we have used a computational model for the fitness landscape of a single fold, built from the observed sequence variation of protein homologues. We have recently shown that such coevolutionary models can be used to design novel foldable sequences. By appropriately combining two of these models to describe the joint fitness landscape of GA and GB, we are able to de

SUBMITTER: Tian P 

PROVIDER: S-EPMC7553338 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature