An optimized protocol for retina single-cell RNA sequencing.

ABSTRACT:

Purpose

Single-cell RNA sequencing (scRNA-seq) is a powerful technique used to explore gene expression at the single cell level. However, appropriate preparation of samples is essential to obtain the most information out of this transformative technology. Generating high-quality single-cell suspensions from the retina is critical to preserve the native expression profile that will ensure meaningful transcriptome data analysis.

Methods

We modified the conditions for rapid and optimal dissociation of retina sample preparation. We also included additional filtering steps in data analysis for retinal scRNA-seq.

Results

We report a gentle method for dissociation of the mouse retina that minimizes cell death and preserves cell morphology. This protocol also results in detec

SUBMITTER: Fadl BR 

PROVIDER: S-EPMC7553720 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature