Negligible transcriptome and metabolome alterations in RNAi insecticidal maize against Monolepta hieroglyphica.

ABSTRACT:

Key message

RNAi-based genetically modified maize resistant to Monolepta hieroglyphica (Motschulsky) was demonstrated with negligible transcriptome and metabolome alterations compared to its unmodified equivalent. As one of the most prevalent insect pests afflicting various crops, Monolepta hieroglyphica (Motschulsky) causes severe loss of agricultural and economic productivity for many years in China. In an effort to reduce damages, in this study, an RNA interference (RNAi)-based genetically modified (GM) maize was developed. It was engineered to produce MhSnf7 double-stranded RNAs (dsRNAs), which can suppress the Snf7 gene expression and then lead M. hieroglyphica to death. Field trail analysis confirmed the robustly insecticidal ability of the MhSnf7 GM maize to resist damages b

SUBMITTER: Zhang X 

PROVIDER: S-EPMC7554010 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature