CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning.

ABSTRACT: The RAG endonuclease initiates Igh locus V(D)J recombination in progenitor (pro)-B cells¹. Upon binding a recombination centre-based J_H, RAG scans upstream chromatin via loop extrusion, potentially mediated by cohesin, to locate Ds and assemble a DJ_H-based recombination centre². CTCF looping factor-bound elements (CBEs) within IGCR1 upstream of Ds impede RAG scanning^3-5; however, their inactivation allows scanning to proximal V_Hs, where additional CBEs activate rearrangement and impede scanning any further upstream⁵. Distal V_H utilization is thought to involve diffusional access to the recombination centre following large-scale Igh locus contraction^6-8. Here we test the potential of linear RA

SUBMITTER: Ba Z 

PROVIDER: S-EPMC7554077 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature