Structural elucidation of triacylglycerol using online acetone Paterno-Buchi reaction coupled with reversed-phase liquid chromatography mass spectrometry.

ABSTRACT: Triacylglycerol (TG) is a class of lipids that is responsible for energy storage and cell metabolism in biological systems; it is found in relatively high abundances in biological fluids such as human plasma. Due to structural complexity, analyzing TGs using shotgun lipidomic approaches is challenging because of the presence of multiple fatty acyl compositional isomers. In this work, reversed-phase liquid chromatography (RPLC) was used for separation of TG species due to the capability of separating lipids based on fatty acyl chain lengths and degrees of unsaturation. RPLC alone does not provide structurally informative information for the location of carbon-carbon double-bonds (C[double bond, length as m-dash]Cs) without using synthesized standards that correspond to each species analyzed

SUBMITTER: Franklin ET 

PROVIDER: S-EPMC7554225 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature