Prolonged fasting drives a program of metabolic inflammation in human adipose tissue.

ABSTRACT:

Objective

The human adaptive fasting response enables survival during periods of caloric deprivation. A crucial component of the fasting response is the shift from glucose metabolism to utilization of lipids, underscoring the importance of adipose tissue as the central lipid-storing organ. The objective of this study was to investigate the response of adipose tissue to a prolonged fast in humans.

Methods

We performed RNA sequencing of subcutaneous adipose tissue samples longitudinally collected during a 10-day, 0-calorie fast in humans. We further investigated observed transcriptional signatures utilizing cultured human monocytes and Thp1 cells. We examined the cellularity of adipose tissue biopsies with transmission electron microscopy and tested for associated changes in r

SUBMITTER: Fazeli PK 

PROVIDER: S-EPMC7554650 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature