Proteomic and Transcriptomic Patterns during Lipid Remodeling in Nannochloropsis gaditana.

ABSTRACT: Nutrient limited conditions are common in natural phytoplankton communities and are often used to increase the yield of lipids from industrial microalgae cultivations. Here we studied the effects of bioavailable nitrogen (N) and phosphorus (P) deprivation on the proteome and transcriptome of the oleaginous marine microalga Nannochloropsis gaditana. Turbidostat cultures were used to selectively apply either N or P deprivation, controlling for variables including the light intensity. Global (cell-wide) changes in the proteome were measured using Tandem Mass Tag (TMT) and LC-MS/MS, whilst gene transcript expression of the same samples was quantified by Illumina RNA-sequencing. We detected 3423 proteins, where 1543 and 113 proteins showed significant changes in abundance in N and P trea

SUBMITTER: Hulatt CJ 

PROVIDER: S-EPMC7554720 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature