Human Natural Antibodies Recognizing Glycan Galβ1-3GlcNAc (Le^C).

ABSTRACT: The level of human natural antibodies of immunoglobulin M isotype against Le^C in patients with breast cancer is lower than in healthy women. The epitope specificity of these antibodies has been characterized using a printed glycan array and enzyme-linked immunosorbent assay (ELISA), the antibodies being isolated from donors' blood using Le^C-Sepharose (Le^C is Galβ1-3GlcNAcβ). The isolated antibodies recognize the disaccharide but do not bind to glycans terminated with Le^C, which implies the impossibility of binding to regular glycoproteins of non-malignant cells. The avidity (as dissociation constant value) of antibodies probed with a multivalent disaccharide is 10^-9 M; the nanomolar level indicates that the concentration is sufficient

SUBMITTER: Dobrochaeva K 

PROVIDER: S-EPMC7554730 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature