Phage Displayed Domain Antibodies (dAb) for Detection of Allergenic Pistachio Proteins in Foods.

ABSTRACT: Pistachio nuts (Pistacia vera) have been consumed by past and present-day civilizations because of their organoleptic characteristics and potential health benefits. However, they can also produce moderate to severe IgE-mediated reactions in allergic individuals. In this work, we report the isolation of the first recombinant antibodies against pistachio nut, produced without animal immunization, to be used in immunoassays for detection of allergenic pistachio in food products. Several phage display biopanning strategies were evaluated to screen the human-based domain antibody library (dAb) in search for pistachio-specific probes. The clone producing the PVF4 phage-dAb was finally selected, and it does not cross-react with cashew despite the phylogenetic proximity with pistachio. West

SUBMITTER: Madrid R 

PROVIDER: S-EPMC7554873 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature