Authentication of Ginkgo biloba Herbal Products by a Novel Quantitative Real-Time PCR Approach.

ABSTRACT: Ginkgo biloba is a widely used medicinal plant. Due to its potential therapeutic effects, it is an ingredient in several herbal products, such as plant infusions and plant food supplements (PFS). Currently, ginkgo is one of the most popular botanicals used in PFS. Due to their popularity and high cost, ginkgo-containing products are prone to be fraudulently substituted by other plant species. Therefore, this work aimed at developing a method for G. biloba detection and quantification. A new internal transcribe spacer (ITS) marker was identified, allowing the development of a ginkgo-specific real-time polymerase chain reaction (PCR) assay targeting the ITS region, with high specificity and sensitivity, down to 0.02 pg of DNA. Additionally, a normalized real-time PCR approach u

SUBMITTER: Grazina L 

PROVIDER: S-EPMC7555165 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature