Diclofenac Degradation-Enzymes, Genetic Background and Cellular Alterations Triggered in Diclofenac-Metabolizing Strain Pseudomonas moorei KB4.

ABSTRACT: Diclofenac (DCF) constitutes one of the most significant ecopollutants detected in various environmental matrices. Biological clean-up technologies that rely on xenobiotics-degrading microorganisms are considered as a valuable alternative for chemical oxidation methods. Up to now, the knowledge about DCF multi-level influence on bacterial cells is fragmentary. In this study, we evaluate the degradation potential and impact of DCF on Pseudomonas moorei KB4 strain. In mono-substrate culture KB4 metabolized 0.5 mg L-1 of DCF, but supplementation with glucose (Glc) and sodium acetate (SA) increased degraded doses up to 1 mg L-1 within 12 days. For all established conditions, 4'-OH-DCF and DCF-lactam were identified. Gene expression analysis revealed the up-regulation of selected genes encoding

SUBMITTER: Zur J 

PROVIDER: S-EPMC7555183 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature