Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins.

ABSTRACT: Aedes aegypti is the primary mosquito vector of several human arboviruses, including the dengue virus (DENV). Vector control is the principal intervention to decrease the transmission of these viruses. The characterization of molecules involved in the mosquito physiological responses to blood-feeding may help identify novel targets useful in designing effective control strategies. In this study, we evaluated the in vivo effect of feeding adult female mosquitoes with human red blood cells reconstituted with either heat-inactivated (IB) or normal plasma (NB). The RNA-seq based transcript expression of IB and NB mosquitoes was compared against sugar-fed (SF) mosquitoes. In in vitro experiments, we treated Aag2 cells with a recombinant version of complement proteins (hC3 or hC5a) and co

SUBMITTER: Giraldo-Calderon GI 

PROVIDER: S-EPMC7555780 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature